Genotyping by Multiplexed Sequencing (GMS) protocol in Barley

Abstract Genotyping by sequencing (GBS) and single nucleotide polymorphism (SNP) chip technologies are the primary SNP genotyping used today. However, these have some drawbacks that limit their usefulness in analysis. We developed a robust protocol called multiplexed (GMS) using markers, providing informative genotypic data with greater flexibility. The genotypes derived from direct sequence reads reduce ambiguity genetic advantages of this include: (1) This PCR-based generates information markers interest provides more streamlined accurate analysis process, multiplexing hundreds into run. (2) marker sets easily customized to species can readily be changed. In study we taken GMS wheat adapted it barley. identified 577 work well adequate genome coverage for genomic selection tag 267 QTL’s genes interest. Good an read depth at least 5 amplicons reliably present across population.